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il 12p70  (R&D Systems)


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    Structured Review

    R&D Systems il 12p70
    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail <t>of</t> <t>IL-12,</t> IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .
    Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12p70/product/R&D Systems
    Average 93 stars, based on 64 article reviews
    il 12p70 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function"

    Article Title: Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function

    Journal: Nature Microbiology

    doi: 10.1038/s41564-024-01838-z

    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .
    Figure Legend Snippet: a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .

    Techniques Used: Incubation, Control, Comparison, Derivative Assay

    Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.
    Figure Legend Snippet: Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.

    Techniques Used: Incubation, Control, Comparison



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    il 12p70  (R&D Systems)
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    R&D Systems il 12p70
    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail <t>of</t> <t>IL-12,</t> IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .
    Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12p70/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    il 12p70 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .

    Journal: Nature Microbiology

    Article Title: Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function

    doi: 10.1038/s41564-024-01838-z

    Figure Lengend Snippet: a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .

    Article Snippet: Surface-enhanced Raman scattering nanotags were conjugated with antibodies against IL-1β (MAB601, clone 2805), IL-6 (AF-206, polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910) or MCP-1 (MAB679, clone 23007) obtained from R&D Systems.

    Techniques: Incubation, Control, Comparison, Derivative Assay

    Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.

    Journal: Nature Microbiology

    Article Title: Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function

    doi: 10.1038/s41564-024-01838-z

    Figure Lengend Snippet: Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.

    Article Snippet: Surface-enhanced Raman scattering nanotags were conjugated with antibodies against IL-1β (MAB601, clone 2805), IL-6 (AF-206, polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910) or MCP-1 (MAB679, clone 23007) obtained from R&D Systems.

    Techniques: Incubation, Control, Comparison